whole mouse genome microarray 4 × 44 slides  (Agilent technologies)

Journal: Nucleic Acids Research

Article Title: Nrf2–MafG heterodimers contribute globally to antioxidant and metabolic networks

doi: 10.1093/nar/gks827

Figure Lengend Snippet: DEM-inducible genes proximal to Nrf2–MafG and Nrf2 single binding sites. Microarray analysis was performed with RNA isolated from DEM- or DMSO-treated Hepa1 cells for 6 h in duplicate. ( A ) Venn diagram showing the overlap of genes induced by DEM (≥1.5-fold change) and genes proximal to Nrf2–MafG or Nrf2 single binding sites. ( B ) Venn diagram showing the overlap of genes repressed by DEM (≥1.5-fold change) and genes proximal to the Nrf2–MafG or Nrf2 single binding sites. ( C , D ) Heat map of differentially expressed genes proximal to the Nrf2–MafG-binding sites (C) or Nrf2 single binding sites (D). DEM-induced genes proximal to Nrf2–MafG-binding sites are categorized into functional groups: antioxidant and detoxification enzymes, proteasome and chaperone, transporter, metabolism and others. The colors of the heat map reflect the log (2) -fold-change values relative to the mean expression level of each gene in the DMSO-treated (Veh) Hepa1 cells. The gene symbols used here are consistent with those used in the Mouse Genome Informatics database.

Article Snippet: The Agilent 4 × 44 K Whole-Mouse Genome Oligo Microarray slides were hybridized, washed and scanned on an Agilent Microarray Scanner according to the Agilent protocol.

Techniques: Binding Assay, Microarray, Isolation, Functional Assay, Expressing